The American superstar will bring the three-day festival to a close tonight – after she was convinced by Coldplay frontman Chris Martin to play – as she headlines the main Pyramid stage.
Jay-Z could make a surprise appearance during her performance, according to the BBC.
She will follow up her Glastonbury performance with a secret gig which will be held tomorrow night.
The Destiny’s Child diva will play to 300 people at a Belvedere Vodka sponsored gig at Shepherd’s Bush Empire to launch her new album 4.
If Jay-Z doesn’t accompany his wife onstage, there’s always a chance that her former Destiny’s Child bandmates will.
Reports have suggested that both Michelle and Kelly are travelling to the festival at Worthy Farm to discuss the possibility of the three of them once again performing together.
Suzanne Vega, the first woman to headline at Glastonbury in 1989, is headlining on the acoustic stage at the same time.
Beyoncé will become part of a very small minority of women who have headlined at the 41-year-old festival.
Beyoncé and Jay-Z were spotted out watching Coldplay perform on Saturday although the Single Ladies singer allegedly avoided the mud by watching from the side of the stage.
She has not worn wellies for the days she has been there and is also planning on getting driven right to the stage tonight to avoid the boggy mess, the Daily Star claims.
Beyoncé is thought to have spent the last three weeks in the UK, rehearsing at a studio in London for her big appearance.